Journal: Nature Communications

Article Title: Extracellular vesicle engineering using a small scaffold protein

doi: 10.1038/s41467-026-70451-x

Figure Lengend Snippet: Engineered EVs displaying species-specific gp130 (mouse mgp130 or human hgp130) were constructed and characterized, confirming protein expression ( a ) and typical EV size distribution ( b ). This figure was created using MedPeer (medpeer.cn). mRNA ( c ) and protein ( d ) levels of TNF-α and IL-6 in RAW 264.7 cells treated with EN144-EV mgp130 post-LPS induction. Error bars were derived from 3 independent experiments. e mRNA expression of IL-6 in liver, spleen, and lung tissues of septic mice 6 h after intravenous injection of 1.0 × 10 10 particles EN144-EV mgp130 . f Expression of FLAG-tagged proteins in cell lysates (top) and EVs (bottom). This figure was created using MedPeer (medpeer.cn). g Size distribution of Expi293F-EVs, EN144-EV mgp130 , and EN144-EV hgp130 . Protein ( h ) and mRNA ( i ) levels of IL-6 in LPS-induced RAW 264.7 cells following co-culture with EN144-EV hgp130 or EN144-EV mgp130 . n = 3 independent experiments. Survival rates of septic mice treated with varying doses of EN144-EV mgp130 ( j ) and EN144-EV hgp130 ( k ). n = 5 mice/group. Survival rates ( l ) and body weight ( m ) changes of septic mice after tail vein injection of EN144-EV mgp130 (1.0 × 10 10 particles/mouse), EN144-EV hgp130 (1.0 × 10 10 particles/mouse), sgp130 (1 µg), or sgp130 (10 µg). n = 5 mice/group. Statistical analysis was performed using the Log-rank (Mantel-Cox) test. Serum levels of inflammatory cytokines and acute-phase proteins in septic mice treated with EN144-EV mgp130 , EN144-EV hgp130 , low-dose sgp130, or high-dose sgp130, including ( n ) IL-6, ( o ) TNF-α, ( p ) IL-1β, ( q ) soluble IL-6 receptor (sIL-6R), ( r ) serum amyloid A (SAA), and ( s ) C-reactive protein (CRP). n = 3 mice/group. The data are presented as mean ± SD. The p values ( c , d , e ) were calculated using a two-tailed t -test. The p values ( i , n , o , p , q , r , s ) were analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons test. * P < 0.05, ** P < 0.01. Source data and exact p value are provided as a file.

Article Snippet: The Rat IL-1β protein (#80023-RNAE) and Mouse gp130/IL6ST protein (50135-M02H) were purchased from SinoBiological, China.

Techniques: Construct, Expressing, Derivative Assay, Injection, Co-Culture Assay, Two Tailed Test

Journal: Advances in Radiation Oncology

Article Title: Kupffer Cell-Derived Interleukin-6 Aggravates Radiation-Induced Liver Disease by Activating Hepatocyte STAT3 to Promote Ccng1 Transcription

doi: 10.1016/j.adro.2026.102003

Figure Lengend Snippet: Schematic of the Kuppfer cell (KC)-hepatocyte crosstalk mechanism in RILD. Irradiation (IR) stimulates KCs to secrete IL-6, which binds to the IL-6R/gp130 complex on hepatocytes to activate JAK; phosphorylated JAK induces STAT3 phosphorylation, and nuclear-translocated p-STAT3 binds to the Ccng1 promoter to promote its transcription; CCNG1 then regulates MDM2 to mediate ubiquitination-dependent TP53 proteolysis, ultimately enhancing hepatocyte apoptosis and driving radiation-induced liver disease (RILD) progression.

Article Snippet: Either a rat interleukin (IL)-6 antibody (anti-IL-6; R&D Systems, #AF506) or soluble rat gp130 Fc chimera protein (sgp130Fc; R&D Systems, #5029-RG-100) was intraperitoneally injected twice weekly at concentrations of 16.7 μg/kg and 0.5 mg/kg, respectively.

Techniques: Irradiation, Phospho-proteomics, Ubiquitin Proteomics